Slicing of a given tissue into thin sections is mandatory for microscopic examination. Sectioning of hard tissues like teeth and bone pose great challenge due to their rigidity. Making ground sections of hard tissues is tedious and time consuming. Decalcification is the only reliable method to remove the inorganic portion of these tissues. However the process of decalcification takes a considerable period of time thereby slowing down the process of reporting hard tissue pathologies. Aim: The study aimed at analyzing the rapidity of decalcification of tooth with varying concentrations of formic acid, nitric acid and EDTA. Materials and Methods: 70 freshly extracted human teeth were divided into 10 different groups and each was treated with varying concentrations of decalcification solution until they were completely decalcified. Results and conclusion: Teeth decalcified with nitric acid solution resulted in rapid decalcification compared to other acids. Teeth treated with EDTA showed very minimal removal of inorganic content over a very long period of time. In routine practice 30% nitric acid can be effectively used for rapid decalcification of teeth in the histopathology laboratory however best staining qualities were noted among the teeth decalcified with 10% nitric acid.
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