Antibacterial Efficacy of Azardirachta Indica (Neem) against oral microorganisms — An in-vitro study

Chole, Revant H; Balsaraf, Swati

doi:10.37841/jidam.2025.v12.i1.002

3

Microorganisms Tested

7

References

2025

Year Published

📝Abstract

Introduction

Neem Tree (Azardirachta indica) is a common evergreen tree with medicinal values. Neem contains the alkaloid margosine, resins, gum, chloride, fluoride, silica, sulfur, tannins, oils, saponins, flavonoids, sterols, and calcium.

Methodology

Antimicrobial susceptibility testing protocols (Schwalve, Moore and Goodwin, CRC Press 2007) were followed after the extract was prepared. The extracts were stored under 5°C and used for Minimum Inhibitory Concentration (MIC) determination in comparison with Chlorhexidine as standard.

Results

The extracts of Neem bark were tested for minimum inhibitory concentration against A. viscosus, L. acidophillus, and S. mutans. Neem bark extracts were most effective against A. viscosus as compared to S. mutans and L. acidophillus.

Conclusion

Neem has many medicinal benefits apart from antibacterial activity. The current study paves the way to study in depth about the antibacterial activity of neem — i.e. how it works at the biomolecular level.

Keywords: Neem BHI Lactobacillus Acidophillus Chlorhexidine Antibacterial

🔖How to Cite

Chole RH, Balsaraf S. Antibacterial Efficacy of Azardirachta Indica (Neem) against oral microorganisms: An in-vitro study. J Indian Dent Assoc Madras. 2025;12(1). DOI: 10.37841/jidam.2025.v12.i1.002

Chole, R. H., & Balsaraf, S. (2025). Antibacterial efficacy of Azardirachta indica (neem) against oral microorganisms: An in-vitro study. Journal of Indian Dental Association Madras, 12(1). https://doi.org/10.37841/jidam.2025.v12.i1.002

Chole RH, Balsaraf S. Antibacterial efficacy of Azardirachta indica (neem) against oral microorganisms: an in-vitro study. J Indian Dent Assoc Madras. 2025;12(1). doi:10.37841/jidam.2025.v12.i1.002

📚References

1
Prashant GM, Chandu GN, Murulikrishna KS, Shafi Ulla MD. The effect of mango and neem extract on four organisms causing dental caries: Streptococcus mutans, Streptococcus salivarius, Streptococcus mitis, and Streptococcus sanguis: An in vitro study. Indian J Dent Res. 2007;18(4).
2
Raut RR, Ajit RS, Bhagyashree BJ. Antimicrobial activity of Azadirachta indica (Neem) against Pathogenic Microorganisms. Journal of Academia and Industrial Research (JAIR). 2014;3(7).
3
Santosh KS, Babita A, Akhilesh K, Archana P. Phytochemicals of Azadirachta Indica Source of Active Medicinal Constituent Used for Cure of Various Diseases: A Review. Journal of Scientific Research. 2020;64(1).
4
Antimicrobial susceptibility testing protocols. Schwalve, Moore and Goodwin. CRC Press; 2007.
5
Bansal V, Gupta M, Bhaduri T, Shaikh SA, Sayed FR, Bansal V, et al. Assessment of antimicrobial effectiveness of neem and clove extract against Streptococcus mutans and Candida albicans: An in vitro study. Niger Med J. 2019;60:285–9.
6
Jain I, Jain P, Bisht D, Sharma A, Srivastava B, Gupta N. Comparative Evaluation of Antibacterial Efficacy of Six Indian Plant Extracts against Streptococcus mutans. J Clin Diagn Res. 2015 Feb;9(2):ZC50–ZC53.
7
Raja RRY, Krishna KC, Lokanatha O, Mamatha S, Damodar. Antimicrobial activity of Azadirachta indica (neem) leaf, bark and seed extracts. Int J Res Phytochem Pharmacol. 2013;3(1):1–4.

[1] 1
Prashant GM, Chandu GN, Murulikrishna KS, Shafi Ulla MD. The effect of mango and neem extract on four organisms causing dental caries: Streptococcus mutans, Streptococcus salivarius, Streptococcus mitis, and Streptococcus sanguis: An in vitro study. Indian J Dent Res. 2007;18(4).

[2] 2
Raut RR, Ajit RS, Bhagyashree BJ. Antimicrobial activity of Azadirachta indica (Neem) against Pathogenic Microorganisms. Journal of Academia and Industrial Research (JAIR). 2014;3(7).

[3] 3
Santosh KS, Babita A, Akhilesh K, Archana P. Phytochemicals of Azadirachta Indica Source of Active Medicinal Constituent Used for Cure of Various Diseases: A Review. Journal of Scientific Research. 2020;64(1).

[4] 4
Antimicrobial susceptibility testing protocols. Schwalve, Moore and Goodwin. CRC Press; 2007.

[5] 5
Bansal V, Gupta M, Bhaduri T, Shaikh SA, Sayed FR, Bansal V, et al. Assessment of antimicrobial effectiveness of neem and clove extract against Streptococcus mutans and Candida albicans: An in vitro study. Niger Med J. 2019;60:285–9.

[6] 6
Jain I, Jain P, Bisht D, Sharma A, Srivastava B, Gupta N. Comparative Evaluation of Antibacterial Efficacy of Six Indian Plant Extracts against Streptococcus mutans. J Clin Diagn Res. 2015 Feb;9(2):ZC50–ZC53.

[7] 7
Raja RRY, Krishna KC, Lokanatha O, Mamatha S, Damodar. Antimicrobial activity of Azadirachta indica (neem) leaf, bark and seed extracts. Int J Res Phytochem Pharmacol. 2013;3(1):1–4.